Saturday, August 10, 2013

Plant Propagation - What is it?

What is Plant Propagation?. It was the basic question when I attended an agriculture basic training course in early days. Plant propagation is the branch of horticulture which deals with the deliberate production of new plants using various starter materials (e.g. organs, tissues) and methods, including their intensive but temporary care. It is primarily practiced to produce seedlings or clones of nursery crops for outplanting, or for planting in containers for display or decor or other uses.  Nursery crops are those which commonly require the use of pre-grown planting materials for outplanting, or field planting. A plant nursery is the place where seedllings, clones, and potted plants are raised temporarily under intensive care. The basis of plant propagation istotipotency, the capability of cells to regenerate missing parts and, subsequently, an entire organism. Applied to plants, it means that any live part that is separated from the parent plant, being composed of cells, can possibly produce missing organs of an intact plant, such as roots and shoot, and give rise to an entire plant. It means that all plant organs with live cells, such as seeds, stems, etc., either intact or segmented, are potential propagules, or propagating materials.  This article I write in "Anim Agro Technology" about what is plant propagation as minor subject but very important in basic agriculture knowledge.

Plant Propagation Methods in general are devided in two (2) methods of propagating plants SEXUAL and ASEXUAL.

Diagram 1: Components of flowers
Sexual propagation is with the use of seed or spore that is separated from the parent plant. This method is so described "sexual" because there is the involvement of the sexes, referring to the contribution of both the male and female gametes in the production of new plants. The young plant that is produced from seed is called seedling. However, this term is now used to refer to any young plant without regard to the method of propagation.  It does not mean, however, that all plants which are propagated by seed are sexually reproduced. There are some exceptions, notably the hawkweed (Hieracium sp.) and farm crops like Mangosteen (Garcinia mangostana) which produce apomictic seeds. Apomixis is the production of seed without sexual union. It also occurs in citrus, lanzones, and polyembryonic mango.

Asexual propagation, also called 'vegetative propagation' is with the use of planting materials which are vegetative parts of any plant rather than seeds or spores which are reproductive parts. In most techniques, the propagule is separated first from the parent plant and treated to induce regeneration or otherwise directly planted. Asexual or vegetative propagation does not involve the union of the male and female gametes. Both meiosis and fertilization are not required in the production of propagules and new plants. Plant propagation involves the use of propagating materials consisting of plant parts which develop through mitotic cell division. As a result, the new plants, called clones, are genotypic duplicates of the mother plants. With the inclusion of apomictic seeds, the following vegetative plant organs are used in natural asexual propagation: bulb and bulblet, clove, corm and cormel, tuber and tubercle, rhizome, stolon, slip, sucker, crown and plantlet.
The following artificial methods of producing clonal seedlings are also employed: propagation by root cutting, stem cutting and leaf cutting, layering and marcotting, budding and grafting.

Tissue culture propagation at lab.
Plant propagation methods can also be divided into two types: Conventional and Modern. Conventional propagation is the usual method of plant multiplication using plant organs, such as seeds and stems, under "outside" or outdoor conditions.  Modern propagation is the rapid multiplication of plants with the use of the same plant organs as well as tissues (example corn embryo, tobacco pith, orchid and banana meristems) and cells as propagule applying advanced techniques like tissue culture. Tissue culture, also called in vitro culture and micropropagation, is the technique of propagating plants indoor under aseptic or sterile conditions in artificial growth media (See photo above). The growth medium, also called nutrient solution, is a mixture of essential elements. The initial propagule in tissue culture is calledexplant.

Seed Propagation and Spore Propagation Different
Seed propagation is applied in the spermatophytes or seed-bearing plants which include the gymnosperms (naked-seed plants) and the angiosperms (enclosed-seed plants or true-flowering plants). Plants which are propagated from spore belong to the fern family and allies, including mosses. In plant propagation using the seed or spore as propagation material, fertilization or the union of the male and female gametes is a precondition for the production of new plants. However, there is a difference between the two. In seeds, a miniature plant (the embryo) within the organ is pre- developed after undergoing fertilization. This embryo is the one which grows to form the new plant.This means that fertilization first occurs (after pollination), and this leads to seed formation. The seed, having a sexually produced and mature embryo, germinates and gives rise to a seedling. But in spore propagation, the spore does not immediately develop into a new plant. The spore is formed without undergoing fertilization. Under moist condition, it develops into a gametophyte or “prothallus,” a flat, green and heart-shaped structure with small rootlike rhizoids. This organ has both male and female gametes which subsequently unite and give rise to new plants. In other words, fertilization comes first, the planting material called seed is formed, and then the seed germinates to form the new plant. But in ferns and other non-seed plants, the planting material called spore is formed first, it develops into a prothallus, fertilization follows, and the fertilized egg develops into a plantlet.


M Anem
Senior Agronomist
Tissue Culture Lab
Chiap Hup Seed Company,
Industrial Park,
Ledang, Johor,
(27 Ramadan 1434H - visit the lab in Dec 2012)

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